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This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.

Biophysics. --- Biomolecules. --- Biomolecules --- Biophysics --- Molecular Biology --- Instruments and instrumentation engineering. --- SCIENCE / Life Sciences / Biophysics. --- TECHNOLOGY & ENGINEERING / Measurement. --- Analysis. --- methods.

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Molecular biology. --- Molecular biology --- Experiments. --- Experiments --- History. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology

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This book presents an overview of the most relevant protein knockdown techniques. Readers will find a description of the generation and use of recombinant human antibodies, ER intrabodies and peptides as well as a description of the working mechanism and potential of the inhibitory action of each of these molecules. The book will also describe the selection and activity of a number of phosphatases, aptamers and allosteric modulators, focusing on A G protein coupled receptors. The book starts with a chapter on the in silico prediction of target-inhibitor interactions. Key aspects of this book are: - Selection strategies for monoclonal and recombinant human antibodies - Selection strategies for ER intrabodies, peptides and aptamers - Examples of the most efficient inhibitors and their applications in protein biochemistry and cell biology - Antibodies for cancer therapy and inhibitors of angiogenesis. The book will be of great interest to scientists and students working in the life sciences on all areas related to protein biology. The variety of methods to modulate the activities of specific proteins which are introduced here will be of great benefit to the reader who is interested in general protein research or to readers who have very specific protein related questions.

Medicine. --- Immunology. --- Biomedicine. --- Proteins --- Synthesis --- Inhibitors. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Immunobiology --- Life sciences --- Serology

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This book reviews the mathematical and statistical calculations related to the day-to-day functions of biotechnology and molecular biology labs, helping students, technicians, and researchers understand which calculations to use and why.

Molecular biology --- Biotechnology --- Biomathematics. --- Mathematics. --- Biology --- Mathematics --- Chemical engineering --- Genetic engineering --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Biotechnology. --- Computer simulation.

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Annette Barchanski deals with the question how to design nanoparticles for biomedical research. She considers properties such as size, charge, biocompatibility, and functionalization of nanoparticles from a biologist’s point of view in order to achieve specific cellular responses. The author discusses the structure-function relationship of nanoparticle conjugates derived from a laser-based fabrication method. Both the limits and perspectives of tunable conjugate functions are presented, providing a general outline for researchers to configure functionalized nanoparticles with a specifically optimized design for biomedical requests, e.g. in biomedical engineering regenerative science and reproductive biology. Contents Nano-Revolution of Biomedical Science and Reproductive Biology Gold Nanoparticles: Optical and Toxicological Aspects; Membrane Interactions; Biological Application Areas Design Criteria and Laser-Based Fabrication of Gold Nanoparticles and Gold Nanoparticle Bioconjugates Yield Enhancement of Laser Ablation Method Structure-Function Relationship: Intrinsic Parameters of Gold Nanoparticles; Conjugation Parameters Laser-Based Fabrication and Bioconjugation of Silicon and Magnetic Nanoparticles Target Groups Researchers and students in the fields of nanotechnology, biomedicine, chemical engineering, laser technology, reproductive biology, and toxicology The Author Annette Barchanski works as a scientific researcher at the Laser Zentrum Hannover e.V.

Chemistry. --- Biomedical engineering. --- Nanochemistry. --- Biophysics. --- Biological physics. --- Biomedical Engineering/Biotechnology. --- Biophysics and Biological Physics. --- Bioconjugates. --- Nanoparticles. --- Clinical engineering --- Medical engineering --- Bioconjugate chemistry --- Conjugated biomolecules --- Conjugation biochemistry --- Conjugation chemistry --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Nanostructured materials --- Particles --- Biomolecules --- Metabolic conjugation --- Biological and Medical Physics, Biophysics. --- Nanoscale chemistry --- Chemistry, Analytic --- Nanoscience --- Analytical chemistry --- Biological physics --- Biology --- Medical sciences --- Physics